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Image Search Results
Journal: Biochemistry and Biophysics Reports
Article Title: PGC-1α attenuates the oxidative stress-induced impaired osteogenesis and angiogenesis regulation effects of mesenchymal stem cells in the presence of diabetic serum
doi: 10.1016/j.bbrep.2021.101070
Figure Lengend Snippet: Protective effect of PGC-1α over-expression on MSCs differentiation in diabetic serum-mediated cellular injury. (A) Relative osteogenic-specific genes RUNX2, Col 1, OCN and OPN mRNA expressions of MSCs. *P < 0.01 and **P < 0.01, vs. Control and PGC-1α Groups, #P < 0.05 vs.DS Group; (B) ALP staining and (D) ALP activity of MSCs after culture for 7 days; (C) ARS staining and (E) quantitative analysis for mineralization assay of MSCs at day 21 were measured; secreted OCN (F) and OPN were measured via ELISA. *P < 0.01 and **P < 0.01, vs. Control and PGC-1α Groups, #P < 0.05 vs.DS Group. Scale bar = 200 μm.
Article Snippet: Osteogenic differentiation medium for human
Techniques: Over Expression, Control, Staining, Activity Assay, Mineralization Assay, Enzyme-linked Immunosorbent Assay